Digital PCR methods potent but not sufficiently robust for RNA virus detection

JRC scientists have assessed the influence of different primer and probe chemistries on the accuracy of digital PCR (dPCR) methods for the quantification of viral Ribonucleic Acid (RNA) using an influenza model. The study demonstrated that innovative reverse transcription digital PCR (RT-dPCR) requires careful evaluation of method performance and quality control, but has, under the condition that it is sufficiently well optimised, large potential for accurate quantification of viral RNA.

Further information: Digital PCR methods potent but not sufficiently robust for RNA virus detection